For gRNA search paste your target region in BED format into the box (example). Target region is defined by TABs delimited chromosome name, start and end position and optionally a target name. Alternatively, you can use the JBrowse for graphical selection of the target region.

Options

Design settings
Off-target settings

off0off1off2off3off4
gRNA scoring

Output
If design settings are OK, please click 'submit job'